Phase two of the Belfast on the Move scheme begins on 28 January.
Many drivers complained of congestion in the city when the first set of lanes were opened last September.
However the Department of Regional Development said traffic flows have since reduced, while bus usage has increased by around 1,500 passengers per day compared to 2011.
"It is expected that, like phase one, there will be a 'bedding in' period for the new works," explained Roads Minister Danny Kennedy.
"We would therefore ask everyone to be patient and allow extra time for their journey."
The second phase of the works will involve changes to lanes in Great Victoria Street, Fisherwick Place, Grosvenor Road, College Avenue, College Square East, Wellington Place, Donegall Square North, Donegall Square East and Chichester Street.
Cycle tracks will be introduced in Victoria Street and Ann Street, and there will be resurfacing work in College Square North, Durham Street and Grosvenor Road.
The work is expected to be finished by the summer.
Mr Kennedy continued: "The aim of Belfast on the Move is to make the city centre more accessible to everyone.
"To achieve this we are encouraging the use of public transport for those who can readily access it and at the same time creating more road space for those who are simply unable to use public transport."