The legendary group will perform 40 shows for the RockTellz & CockTails concert series, starting on 20 February.
A statement from singer Jackie Jackson read: "We are thrilled to be a part of RockTellz & Cocktails at Planet Hollywood. As a Las Vegas resident, I couldn't be more proud to be a part of this amazing series at one of the most iconic entertainment destinations in the world.
"My brothers and I can't wait to take the stage and bring our hits - and the stories behind them - to life for the fans."
They will rotate with singer Meat Loaf, who debuted his show last autumn and will return to the Vegas stage in February.
The Jacksons reunited in 2012, three years after the death of Michael Jackson, after a 30-year hiatus.