In a surprise result of the public vote the actor faced off against series favourite Ray Quinn in the skate-off, but unfortunately lost his place after all four of the judges felt Ray should progress to the final.
After their skate-off routines, judge Karen Barber told them: "To me you are both champions."
Jason Gardiner added: "Neither of you should be here, I do not agree with this decision."
Speaking to hosts Phillip Schofield and Christine Bleakley, Sam said: "It is my time to go, I have been in too many skate-offs now."
Referring to his skating partner and real-life fiancée Vicky Ogden, whom he met on the show, he added: "I feel like I am the winner here. I have something so much more than a trophy."
Despite his exit from the show is was a positive night for Sam, who scored very high marks from the judges.
He and Vicky opened the show with an energetic performance to Robbie Williams' Let Me Entertain You, which Ashley Roberts described as "fun, fast and furious".
Robin Cousins said: "With that routine you really did come out of the gate with full force - that was absolutely on the money."
That was explosive and energetic!
Jason Gardiner to Sam and Vicky
The pair performed a second routine which required Sam to 'fly' with the use of wires and earned 36 points out of a possible 40.
It was also a winning night for Coronation Street actress Hayley Tamaddon, who earned her first perfect score of the series with her flying routine.
The emotional performance reduced the panel of judges to tears, with Karen telling her: "That was the most beautiful flying routine we have ever seen on Dancing On Ice. You really made us feel it."
Robin said: "That was exquisite to watch."
Hayley's high scores continued during her second performance, earning 39 points and placing her and partner Dan Whiston at the top of the leaderboard.
Meanwhile Olympic gymnast Beth Tweddle returned to the ice this week after battling a virus.
She and partner Lukasz Rosycki scored 36.5 points for their first performance, while they received 37 points after their flying routine.
But while the judges praised Beth for her great technical abilities, Jason once again commented that she was not showing enough personality on the ice compared to her fellow contestants who are all actors.
You attacked that performance really well, you have great technical skills.
Robin Cousins to Beth Tweddle
"I saw more personality in your pre-performance VT than I did on the ice," Jason told her.
But despite the criticism Beth was voted straight through to next week's final - along with Hayley - leaving Ray Quinn to face his first ever skate-off.
Ray has been the favourite to lift the champion of champion's trophy throughout the series, but his uneasiness during his flying routine was noted by the judges.
Scoring 37 points, Robin told him: "It just wasn't all quite there I'm afraid."
Jason added: "I was not moved by that performance, to be honest it left me feeling a little bit cold."
However Ray and partner Maria Filippov managed to return to their former glory by pulling in a perfect score for their second performance.
Karen described their routine as "absolutely beautiful" while Ashley told them it was "always a joy" to watch them performance.
The Dancing On Ice final airs on UTV on Sunday from 6.15pm.