The pop superstar uploaded a snap of her teen son Rocco fighting in the boxing ring on the picture-sharing website on Friday alongside the caption, "No one messes with Dirty Soap! Mama said knock you out! #disn****."
After she was bombarded with complaints about use of the N-word from fans, she deleted the image, only to re-upload it with another risque comment.
She added: "Ok let me start this again. #get off of my d*** haters!"
It's not the first time this month the Material Girl has caused a stir after sharing photos of her eldest son - she came under fire over the New Year when the 13-year-old and his pals posed for a snap holding bottles of alcohol.
Critics questioned Madonna's parenting skills online, prompting her to hit back, writing: "No one was drinking we were just having fun! Calm down and get a sense of humour! Don't start the year off with judgement!"
Rocco is Madonna's only child with ex-husband Guy Ritchie. She is also mum to teenage daughter Lourdes, from a previous relationship, and adopted kids Mercy and David.