Five key members of the cast will be in Derry~Londonderry, the UK City of Culture, for a five-day stint in the city.
"Hollyoaks is known for its exciting storylines that often take viewers outside of the village," a spokesperson for the show's producers, Lime Pictures, said.
"We are looking forward to shooting in Derry."
The Hollyoaks stars will be filming at some of the most prominent, recognisable local landmarks from Monday through to Friday.
"Having Hollyoaks in town will provide a great platform for showing Derry~Londonderry to a new young audience," Shona McCarthy, Chief Executive of Culture Company 2013, said.
"The city is looking so fantastic at the minute, I'm sure it's going to shine like a real star of the show when the storyline airs on Channel 4 later this autumn."
The shoot has been funded by the Arts Council NI, through Northern Ireland Screen.