Declan Fitzpatrick, Niall O'Connor and Darren Cave all coming into the starting 15 for the PRO12 fixture.
The Ulster coach has also been boosted by the return of Ireland Internationals Andrew Trimble and Stephen Ferris who are named on a vastly experienced replacements bench.
The only change up front sees Fitzpatrick replace John Afoa at tighthead prop, where he is joined in the front row by hooker Rob Herring and Callum Black.
Captain Johann Muller and Lewis Stevenson continue in the second row, while the loose forward trio of Sean Doyle, Robbie Diack and Nick Williams will be hoping to continue their impressive form from last week.
With Paddy Jackson still not fully recovered from his calf injury, O'Connor will start ahead of Paddy Jackson at flyhalf, with Michael Heaney retaining the number 9 jersey.
Darren Cave has been promoted from the bench to start at outside centre at the expense of Chris Farrell. Luke Marshall, Michael Allen, Craig Gilroy and Jared Payne complete the backline.
Ulster will be hoping to improve on their away game after two defeats at Connacht and Minster.
The Ulstermen have won three of their last four encounters against Welsh regions, but have lost on their last four visits to the Principality.
Ospreys' seven game winning streak in the PRO12 ended in Italy last Friday, with a surprise 6-12 reversal to Benetton Treviso.
The Welsh team have won their last three matches at Liberty Stadium and lost only once to an Irish province last season: 14-15 to Ulster at Ravenhill on 24 February.
The last four fixtures between the two sides have all been won by the home side on the day, whilst Ulster's only victory at Liberty Stadium since 2006 was 20-16 in September 2009.
Anscombe said he was delighted to welcome back Ferris and Trimble to his match day squad: "When you bring players of that calibre back into the mix it gives everyone a lift.
"The two boys haven't played much rugby in a while so you have to put that in perspective and not expect too much from them this early. Putting them on the bench is a good way of easing them back in," he said.
"Darren Cave was on the bench last week, he's been training with the team now for a few weeks and I though he came on for the last quarter of an hour last week and added greatly to the team.
"He's an International so he brings that experience to a youthful backline. Declan is the same, he's been training well and we wanted to have a look at him from the start."
"There are a lot of areas to improve on but you would expect that and the main thing is that we're progressing," the coach explained.
"Over the next few weeks we'll keep integrating players back into the team and that will allow us to keep developing and growing our game. Its early days and we've just got to keep our feet on the ground and know where we're at."
Ospreys v Ulster (Liberty Stadium, 6.30pm)
(15-9): J Payne; C Gilroy, D Cave, L Marshall, M Allen; N O'Connor, M Heaney;
(1-8): C Black, R Herring, D Fitzpatrick, J Muller (c), L Stevenson, R Diack, S Doyle, N Williams;
N Brady, T Court, J Afoa, N McComb, S Ferris, P Marshall, P Jackson, A Trimble.