The game against England Knights will be the first international rugby league match in Belfast since the 2000 World Cup when Ireland beat Samoa.
Ireland coach Mark Aston has included 13 uncapped players in his initial 30-man squad for his side's Alitalia Cup matches against Scotland and England Knights.
The squad sees a healthy mix of players from Ireland's domestic rugby league competition as well as a number of players from England's Super League - one of the world's two big rugby league competitions.
Training and taking the field alongside players from Wigan Warriors, Warrington Wolves and Leeds Rhinos - the latter two of which will contest the Super League Grand Final at Old Trafford on Saturday - are men who play their club games for teams like Antrim Eels, Athboy Longhorns and the Dublin City Exiles.
The England Knights squad features a host of names taken from Super League, including Liam Farrell of Wigan Warriors, Bradford Bulls' Tom Burgess - who will spend next year in the glamorous NRL, Australia's top rugby league competition, playing for Russell Crowe's south Sydney Rabbitohs team - and Warrington Wolves' Richie Myler and Stefan Ratchford.
The match is part of both England and Ireland's preparation for the 2013 Rugby League World Cup, which will be held in England and Wales - with the Irish side also playing next week against Scotland in Edinburgh on October 14 as part of the three-team 2012 Alitalia European Cup.
Announcing the squad, Aston said: "I am looking forward to this year's Alitalia European Cup as we are bringing through some new faces who will get their chance to showcase their skills in a good tournament."
"We have recently had tough matches against Scotland and England Knights so we are hoping to improve on those performances as we continue to prepare for the 2013 World Cup.
"It's great to be able to call up new domestic players who have played well for their provinces and for Ireland 'A'. This is a good chance for them to be part of the senior squad and show me what they are about."
Ireland have won three and lost two of their last five meetings with Scotland, and will be hoping to maintain that recent winning record with another victory.
Their only ever game against the England Knights was on June 16, in Sheffield, and they lost 62-4 - but they are confident that the Belfast match will be much more competitive.
Gordon Matthews, General Manager of Rugby League Ireland, said: "The game against England Knights will be the first game in Belfast for twelve years so we are excited to be bringing the Alitalia European Cup to Ulster."
The game kicks off at 6pm on the new date of Saturday, October 20 at Deramore Park.
Full squad: M Hadden (Antrim Eels), S Deevy (Athboy Longhorns), W Kerr (Country Cowboys), E Cosgrove (Dewsbury), T McKeown, J Taylor (Dublin City Exiles), L Finn (Featherstone, capt), L Ambler, C Casey, P Handforth, S Hesketh (all Halifax), C Roache (Leeds), T Goulden, S Littler, G McNally (all Leigh), J Toole (Mounties), J Mulhern (Oldham), T Bergin, P Smith (Sheffield Eagles), B Guilfoyle, A McCloskey (Treaty City), K Haggerty (Widnes), J Hinchcliffe, S Wellens (both unattached), D Bridge, J Mendeika, T McCarthy (all Warrington), M Haggerty, C Sice (Whitehaven), J O'Donnell (Wigan).