The Dungiven southpaw saw his world title dreams ended prematurely at the MEN Arena in Manchester back in April, after an accidental clash of heads with Khan opened a gash above his eyebrow, causing the match doctor to controversially intervene and call a halt to the contest.
His opponent for Saturday's clash at the Odyssey also faced Khan but with differing fortunes, beating the Bolton fighter with a stunning first round knockout - and McCloskey has no intention of taking the challenge of the big-hitting south American lightly.
"I'm always confident but don't get me wrong, I'm not underestimating my opponent", McCloskey told UTV.
"I know what this fella brings to the table. He is a tough, strong boy."
"I've studied him, and he is very dangerous early on in a fight, very dangerous over the first four or five rounds.
"He sort of plateaus after that, so we have a game-plan."
As much as both men involved in Saturday's contest have put Khan to the back of their minds, the same could not be said about the assembled media, who were keen to ask about the possibility of a rematch.
"All through our press conference we talked nearly more about Amir Khan than about ourselves because we both fought him.
"He (Prescott) knocked him out, I had the decision that I had against him, which obviously I feel was controversial with the way the stoppage happened but it happened and it's gone now so we are both linked to him in some way.
"But this is not about Amir Khan. It is about being a world champion.
"If it's Khan, beautiful, but if it's not and it's someone else, fine, I don't really care.
"Khan is always going to be linked with me, because of what happened over there, but I don't really care.
"He's moved on, I've moved on and I'm ready to push on quickly and fight for a world title."
Also on Saturday night's bill, Belfast super-bantamweight Carl Frampton faces Mark Quon for the vacant Commonwealth title.
Quon was a late replacement for Frampton's original opponent, European champion Kiko Martinez, who was forced to pull out of the bout for family reasons.
However, the Tigers Bay man is still expecting a tough time against the Australian, although there is still some lingering disappointment that his clash against Martinez fell through.
"That was the fight we wanted", Frampton admitted.
"Martinez is a great fighter, a big puncher, and I think our styles would have gelled well and made an explosive fight.
"But fair play to this lad Mark Quon, he took the fight on short notice and he is here to win."
"We had prepared long and hard for Kiko Martinez, all my sparring partners were fitted around his style.
"But I have had a lot of amateur fights, I have fought against every single style that there is in the world, and I don't think this lad is going to bring anything that I haven't seen before, so it is up to me to deal with it."