Published Tuesday, 11 September 2012
Andy LIttle of Rangers and Northern Ireland. (© Pacemaker)
In a statement, the IFA said the Rangers man has returned to his club and no replacement players will be called in for the fixture at Windsor Park.
Little, who can play a variety of positions, was under consideration to start on the right side of midfield - and has been in great form at Ibrox this season.
The game will kick off at 7.45pm as Northern Ireland look to bounce back from last Friday's 2-0 defeat to Russia, ahead of a tricky trip to Portugal next month.
Little, who opted not to leave Rangers following its financial collapse and re-establishment in the Irn Bru Third Division, came off the bench to play 15 minutes of the defeat to Russia.
He has been a prolific scorer for the Scottish side this season, netting eight goals from eight games from midfield.
The 23-year-old was hopeful of being named as a starter to play in a more attacking formation against Luxembourg, one of group F's more winnable ties.
Little's absence will raise Corry Evans' hopes of keeping his place in the side, however other options for boss Michael O'Neill include a returning Shane Ferguson return on the left, with Chris Brunt on the right wing.