Organisers revealed on Tuesday that more than 17,500 people registered to take part in the race will pause in memory of three spectators, including an eight-year-old boy, who were killed in the double bomb attack in the US city.
More than 250 other people were injured in the explosions close to the finishing line of the Boston Marathon earlier this month.
A 26-year-old man, Tamerlan Tsarnaeve, was killed during a police shoot-out days after the attack, while his 19-year-old brother has been charged in connection with the blasts.
Participants will run, walk and wheel through Belfast during Monday's marathon, with strong competition from former Olympic gold medallist and world champion runner Hunde Gezahegne Abere.
The Ethiopian will be hoping to come in ahead of fellow countrymen Wosen Zeleke Demse, Ashenafi Eshetu Deme and Birhanu Niguse Melese.
Lemlem Berbe Yachem will be the woman to beat on the day, as the 21-year-old tries to improve on her personal best of two hours 34 minutes and 38 seconds.
The leading local runner is Irish international Thomas Frazer, from St Malachy's Athletics Club, who will be trying to improve his personal best time of two hours 18 minutes and 37 seconds.