The British beauty donned the company's iconic Playboy bunny outfit for a series of saucy snaps in celebration of her 40th birthday.
The shoot coincided with the magazine's 60th anniversary, and an image of Moss posing in the sexy ensemble on all fours has been a big hit online.
However, Playboy executives have accused chiefs at Harper's Bazaar's publisher, Hearst Communications, of using the snap, and a series of other pictures from the shoot, online without permission.
In legal papers filed at Manhattan federal court in New York on Friday, lawyers for the plaintiffs claim Harper's Bazaar editors posted the clothed snap of the star, but also included a link to another website which featured Moss' naked images, breaking copyright rules.
Playboy is seeking $150,000 (£93,700) for the alleged copyright infringement of each photo.
Moss hit the age milestone on Thursday and celebrated with a star-studded lunch in London.