The McFly star attended the ceremony at the O2 Arena in London with his bandmates Harry Judd, Dougie Poynter and Danny Jones and spent much of the evening looking for his other band - the one on his finger.
The singer immediately took to his Twitter.com page to alert followers and fellow guests at the event to his plight, even posting a snap of his ring-free left hand.
He posted: "At the NTAs and have discovered that my wedding ring is not on my finger……er?!?!... Dearest people at the NTAs at the O2, please keep a lookout for my wedding ring! I’d really like it back!"
Fletcher wed his long-time girlfriend Giovanna Falcone in May.