The hip-hop duo will take to the stage on Thursday 10 July, alongside special guest Ellie Goulding.
The gig announcement comes after Macklemore & Ryan Lewis won four Grammy Awards, including best rap album, on Sunday.
The pair also performed their hit song Same Love at the star-studded event in Los Angeles as a mix of 33 gay and straight couples were married live on stage by Queen Latifah.
Macklemore & Ryan Lewis skyrocketed to fame with the release of their smash hit Thrift Shop and more recently they have broken new ground by independently releasing their debut full-length album, The Heist, which shot number one on the iTunes chart.
The gig announcement comes after UK band Arctic Monkeys revealed they will be playing at Marlay Park on Saturday 12 July, with special guest Jake Bugg.
Tickets for Macklemore & Ryan Lewis go on sale Saturday 1 February from 10am.