Despite receiving positive feedback from the judging panel for their rendition of Bruno Mars' 'Marry You', which included a routine with five 'brides' onstage, the boyband failed to get enough public votes on Saturday night.
Following their performance, Conor, Josh, Jay, Matt and Thompson received high praise from their mentor Gary Barlow, who told them: "You're work ethic is unbelievable."
He added: "When I arrive at the house on a Monday morning (you) have got (your) harmonies all ready, (you've) decided who is singing what.
"Your worth ethic is what is going to pay off for your guys in the competition."
Louis said: "You were good last week, you were great this week.
"Gary and I know more about boybands than anyone else on this panel - we think you've got something special."
Sharon Osbourne was also impressed by the boys, commenting that they "light up the room" whenever they perform.
But while fellow panelist Nicole Scherzinger praised Kingsland Road, she felt their performance could have used more of a lead vocal rather than all five boys harmonising throughout the song.
I loved the staging, I loved the styling and I loved your energy.
Louis Walsh to Kingsland Road
"We have got 11 lead vocals in the competition, the guys wanted to sing together this week as a vocal harmony group," Gary told her.
The group was visibly shocked when they were told they would be performing in Sunday's sing-off, with band member Conor telling host Dermot O'Leary: "We were seriously nervous about performing tonight. We've worked so hard and love going out on that stage so much, so we hoped it would pay off. We can only give it our all and we really did."
With Miss Dynamix unable to perform during Saturday's live show, the remaining nine acts also took to the X Factor stage to perform in the 'Love and Heartache' themed show.
Fan favourite Sam Bailey opened the programme with her rendition of the Bob Dylan classic 'Make You Feel My Love', which was famously covered by Adele.
Gary told her: "You are really taking the bar to another level with that performance."
But Nicole was the only judge who was not impressed by Sam's efforts, describing her performance as "lukewarm".
Birthday girl Shelley Smith, who was in last week's bottom two following the flash vote, performed Beyonce's 'Single Ladies' for the judges, though they felt it was not the best song choice for her.
Tamera Foster, 16, impressed the judges with her performance of Labrinth's 'Beneath Your Beautiful', while Nicholas McDonald's rendition of Robbie Williams' 'She's the One' led Louis to describe him as a "baby Buble".
I am on the edge of my seat when I am watching you.
Gary Barlow to Hannah Barrett
Supermarket worker Abi Alton, 19, had mixed reviews from the panel following her rendition of Kylie's 'Can't Get You Out of My Head, with Sharon Osbourne telling her she needed more confidence in her performance.
Meanwhile Louis' remaining boys Luke Friend, 17, earned praise for Passenger's 'Let Her Go' and Sam Callahan, 19, performed Jason Mraz's 'I Won't Give Up'.
Rough Copy sang the Backstreet Boys classic 'I Want It That Way', with Gary describing them as "the surprise of the competition so far".
Closing the second live show was A-Level student Hannah Barrett with a rendition of Christina Aguilera's 'Beautiful' that brought mentor Nicole to her feet.
"That was absolutely magnificent," Sharon told her. "You are beautiful inside and out."
Gary said: "This is where you are at your best, when you are singing for your life and I love it because I am on the edge of my seat when I am watching you.
"What a vocal tonight, absolutely extraordinary."
Nicole told her she gave her "face goosebumps", while Louis agreed it was a "powerful" performance.
Who will join Kingsland Road in the sing off? Find out during the results show on Sunday on UTV from 8pm.