The Hollywood beauty claimed the first three prizes of Wednesday's ceremony as she picked up the title for Favorite Movie Actress and was handed trophies for Favorite Comedic and Dramatic Movie Actress, too.
Her space disaster film, Gravity, was also named Favorite Dramatic Movie and her onscreen work with George Clooney landed her the Favorite Movie Duo award.
Timberlake was a triple threat at the Los Angeles ceremony, claiming Favorite Male Artist, R&B Artist and Favorite Album for The 20/20 Experience.
Robert Downey, Jr. was another big winner - he walked away as the Favorite Action Movie Star, while his superhero blockbuster Iron Man 3 was named Favorite Movie and Favorite Action Movie.
Newlywed Kaley Cuoco was given another couple of reasons to celebrate - she was handed the Favorite Comedic TV Actress honour for The Big Bang Theory, while the sitcom was also the winner of the Favorite Network TV Comedy category.
As she accepted her acting accolade, Cuoco, who wed tennis player Ryan Sweeting on New Year's Eve, gave a special shout out to her new husband, saying: "This is the second best thing that happened to me all year; the first was marrying you baby! I love you."
Meanwhile, Jennifer Hudson fought back tears as she was hailed the Favorite Humanitarian for her work with the Julian D. King Gift Foundation, the children's organisation she founded in 2008 to help underprivileged kids following the murder of her seven-year-old nephew.
She stepped onstage with her sister Julia to accept the award and paid tribute to her late mother and brother, who were also killed in the family tragedy, telling the crowd: "We wanna dedicate this to our mother, who taught us... that without family, you have nothing, and our brother, who was the most giving."
Awards co-hosts Kat Dennings and Beth Behrs kicked off the show with a skit straight from the diner of their hit sitcom 2 Broke Girls, featuring guest appearances by The Vampire Diaries' Ian Somerhalder, The Crazy Ones' Sarah Michelle Gellar, Christina Aguilera, Ian Ziering, The Big Bang Theory co-stars Mayim Bialik and Melissa Rauch, and talk show host Arsenio Hall.
Cuoco also made a cameo in the opening sketch, handing over the honour of presenting the prizegiving after joking that she was going to be busy on the night of the event.
Musical performances came from the likes of Brad Paisley and OneRepublic, and presenters included LL Cool J, Zac Efron, Heidi Klum, Jessica Alba and Malin Akerman.
Full list of winners:
- Favorite Movie - Iron Man 3
- Favorite Movie Actor - Johnny Depp
- Favorite Movie Actress - Sandra Bullock
- Favorite Movie Duo - Sandra Bullock and George Clooney (Gravity)
- Favorite Action Movie - Iron Man 3
- Favorite Action Movie Star - Robert Downey, Jr.
- Favorite Comedic Movie - The Heat
- Favorite Comedic Movie Actor - Adam Sandler
- Favorite Comedic Movie Actress - Sandra Bullock
- Favorite Dramatic Movie - Gravity
- Favorite Dramatic Movie Actor - Leonardo DiCaprio
- Favorite Dramatic Movie Actress - Sandra Bullock
- Favorite Family Movie - Despicable Me 2
- Favorite Horror Movie - Carrie
- Favorite Thriller Movie - Now You See Me
- Favorite Network TV Comedy - The Big Bang Theory
- Favorite Comedic TV Actor - Chris Colfer (Glee)
- Favorite Comedic TV Actress - Kaley Cuoco (The Big Bang Theory)
- Favorite Network TV Drama - The Good Wife
- Favorite Dramatic TV Actor - Josh Charles (The Good Wife)
- Favorite Dramatic TV Actress - Stana Katic (Castle)
- Favorite Actor in a New TV Series - Joseph Morgan (The Vampire Diaries)
- Favorite Actress in a New TV Series - Sarah Michelle Gellar (The Crazy Ones)
- Favorite TV Crime Drama - Castle
- Favorite Male Artist - Justin Timberlake
- Favorite Female Artist - Demi Lovato
- Favorite Breakout Artist - Ariana Grande
- Favorite Pop Artist - Britney Spears
- Favorite Country Artist - Taylor Swift
- Favorite Country Music Icon - Tim McGraw
- Favorite Hip Hop Artist - Macklemore & Ryan Lewis
- Favorite R&B Artist - Justin Timberlake
- Favorite Band - One Direction
- Favorite Song - Roar by Katy Perry
- Favorite Album - Justin Timberlake (The 20/20 Experience)
- Favorite Music Video - Roar by Katy Perry
- Favourite Humanitarian - Jennifer Hudson.